Tuesday, March 22, 2016

Homogenizing from A - Z Post #4

Homogenizing A - Z 


Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters H & I here: 

Hair shampoo
PRODUCT APPLICATION REPORT
MATERIAL:Hair shampoo
VOLUME OF MATERIAL:15000-20000g
MEDIUM:None
VOLUME OF MEDIUM:None
PROCESS:Mix shampoo to desired consistency
PREVIOUS OR CURRENT METHOD:Large mixer with 24" rotor and
4" blade
EQUIPMENT USED:PRO250 Homogenizer w/stand & 20mm probe or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS:The blade moved a great deal of the
shampoo because the high rpms.

Hamster brain
PRODUCT APPLICATION REPORT
MATERIAL:Hamster brain
VOLUME OF MATERIAL:10 ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization for RNA and protein purification
analysis
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Successful completion of application.

Hamster tissue
PRODUCT APPLICATION REPORT
MATERIAL:Hamster tissue
VOLUME OF MATERIAL:9 grams
MEDIUM:Aqueous
VOLUME OF MEDIUM:1-5 ml
PROCESS:Release bacteria from tissue
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Required results achieved easily.

Heart cells
PRODUCT APPLICATION REPORT
MATERIAL:Heart cells
VOLUME OF MATERIAL:Not reported
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Isolation of metabolites
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Worked well. Intended results achieved
within seconds.

Hippocampus
PRODUCT APPLICATION REPORT
MATERIAL:Rat hippocampus
VOLUME OF MATERIAL:Not reported
MEDIUM:Cocktail lysis buffer containing protease
inhibitors and phosphatase inhibitors
VOLUME OF MEDIUM:Not reported
PROCESS:Snap-frozen in liquid nitrogen and homogenized
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer 
APPLICATION AND RESULTS:
DOI:		Achieved desired homogenization results
http://dx.doi.org/10.1016/j.neuropharm.2014.10.011


Hippocampus and prefrontal cortex 
PRODUCT APPLICATION REPORT
MATERIAL:Hippocampus and prefrontal cortex 
VOLUME OF MATERIAL:1 ml
MEDIUM:
VOLUME OF MEDIUM:Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO Homogenizer
APPLICATION AND RESULTS:

DOI:		Hippocampus and prefrontol cortex and
1.0ml for nucleus accumbens, using a handheld homogenizer on slow setting.
http://dx.doi.org/10.1007/s00216-013-7514-9

HIV Infected H9 cells
PRODUCT APPLICATION REPORT
MATERIAL:HIV Infected H9 cells
VOLUME OF MATERIAL:20-200 g
MEDIUM:Not reported
VOLUME OF MEDIUM:50-400 ml
PROCESS:Disrupt cell membrane
PREVIOUS OR CURRENT METHOD:Dounce Homogenizer
EQUIPMENT USED:PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed chamber assembly
APPLICATION AND RESULTS:Worked well and sealed system is a
must. However, sharpened blades may pose a huge health risk if
poked into skin.

RECOMMENDATIONS: As an added safety feature for working with a
such material, we would suggest the use of a generator system
(smooth bottomed) that has no exposed sharp edges that can break
the skin of its user.

Human liver
PRODUCT APPLICATION REPORT
MATERIAL:Human liver
VOLUME OF MATERIAL:100-200 ml
MEDIUM:Water
VOLUME OF MEDIUM:Not reported
PROCESS:Complete homogenization
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and blade and probe sealed chamber assembly and 20mm probe 
APPLICATION AND RESULTS:Worked best with the blade and sealed
chamber assembly. Chunks were too big for the 20mm generator.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL:Human tissue
VOLUME OF MATERIAL:5 g
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Brinkmannn homogenizer
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Homogenized tissue well and was quieter
than previous methods.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL:Human tissue
VOLUME OF MATERIAL:.05 ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenize RNA
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Worked well

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL:Human tissue
VOLUME OF MATERIAL:8 ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:RNA extraction
PREVIOUS OR CURRENT METHOD:Brinkmannn Polytron
EQUIPMENT USED:PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS:Liked low noise level of units. Achieved
desired results.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL:Human tissue
VOLUME OF MATERIAL:20 ml
MEDIUM:Phosphate buffer
VOLUME OF MEDIUM:50 ml
PROCESS:Complete homogenization
PREVIOUS OR CURRENT METHOD:shift blender, mortar and pestle
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Worked very well and desired results were
achieved.

Intestinal Samples (Swine)
PRODUCT APPLICATION REPORT


MATERIAL:Intestinal samples (Swine)
VOLUME OF MATERIAL:2mm pieces
MEDIUM:
VOLUME OF MEDIUM:
PROCESS:Lysed
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:PRO200 Homogenizer
APPLICATION AND RESULTS:


DOI:		To obtain representative measurements from the
2-cm jejunal samples, three cross-sections of
approximately 2 mm were pooled and lysed using
PRO 200 Post-Mounted Laboratory

http://dx.doi.org/10.1371/journal.pone.0079343


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