Homogenizing from A - Z Post #4

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters H & I here: 

Hair shampoo

PRODUCT APPLICATION REPORT

MATERIAL: Hair shampoo VOLUME OF MATERIAL: 15000-20000g MEDIUM: None VOLUME OF MEDIUM: None PROCESS: Mix shampoo to desired consistency PREVIOUS OR CURRENT METHOD: Large mixer with 24" rotor and 4" blade EQUIPMENT USED: PRO250 Homogenizer w/stand & 20mm probe or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: The blade moved a great deal of the shampoo because the high rpms.

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Hamster brain

PRODUCT APPLICATION REPORT

MATERIAL: Hamster brain VOLUME OF MATERIAL: 10 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization for RNA and protein purification analysis PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Successful completion of application.

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Hamster tissue

PRODUCT APPLICATION REPORT

MATERIAL: Hamster tissue VOLUME OF MATERIAL: 9 grams MEDIUM: Aqueous VOLUME OF MEDIUM: 1-5 ml PROCESS: Release bacteria from tissue PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Required results achieved easily.

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Heart cells

PRODUCT APPLICATION REPORT

MATERIAL: Heart cells VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Isolation of metabolites PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Worked well. Intended results achieved within seconds.

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Hippocampus PRODUCT APPLICATION REPORT MATERIAL: Rat hippocampus VOLUME OF MATERIAL: Not reported MEDIUM: Cocktail lysis buffer containing protease inhibitors and phosphatase inhibitors VOLUME OF MEDIUM: Not reported PROCESS: Snap-frozen in liquid nitrogen and homogenized PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer  APPLICATION AND RESULTS: DOI: Achieved desired homogenization results http://dx.doi.org/10.1016/j.neuropharm.2014.10.011 Hippocampus and prefrontal cortex

PRODUCT APPLICATION REPORT

MATERIAL: Hippocampus and prefrontal cortex  VOLUME OF MATERIAL: 1 ml MEDIUM: VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO Homogenizer APPLICATION AND RESULTS: DOI: Hippocampus and prefrontol cortex and 1.0ml for nucleus accumbens, using a handheld homogenizer on slow setting. http://dx.doi.org/10.1007/s00216-013-7514-9

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HIV Infected H9 cells

PRODUCT APPLICATION REPORT

MATERIAL: HIV Infected H9 cells VOLUME OF MATERIAL: 20-200 g MEDIUM: Not reported VOLUME OF MEDIUM: 50-400 ml PROCESS: Disrupt cell membrane PREVIOUS OR CURRENT METHOD: Dounce Homogenizer EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed chamber assembly APPLICATION AND RESULTS: Worked well and sealed system is a must. However, sharpened blades may pose a huge health risk if poked into skin. RECOMMENDATIONS: As an added safety feature for working with a such material, we would suggest the use of a generator system (smooth bottomed) that has no exposed sharp edges that can break the skin of its user.

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Human liver

PRODUCT APPLICATION REPORT

MATERIAL: Human liver VOLUME OF MATERIAL: 100-200 ml MEDIUM: Water VOLUME OF MEDIUM: Not reported PROCESS: Complete homogenization PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and blade and probe sealed chamber assembly and 20mm probe  APPLICATION AND RESULTS: Worked best with the blade and sealed chamber assembly. Chunks were too big for the 20mm generator.

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Human tissue

PRODUCT APPLICATION REPORT

MATERIAL: Human tissue VOLUME OF MATERIAL: 5 g MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Brinkmannn homogenizer EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Homogenized tissue well and was quieter than previous methods.

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Human tissue

PRODUCT APPLICATION REPORT

MATERIAL: Human tissue VOLUME OF MATERIAL: .05 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenize RNA PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Worked well

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Human tissue

PRODUCT APPLICATION REPORT

MATERIAL: Human tissue VOLUME OF MATERIAL: 8 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: RNA extraction PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Liked low noise level of units. Achieved desired results.

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Human tissue

PRODUCT APPLICATION REPORT

MATERIAL: Human tissue VOLUME OF MATERIAL: 20 ml MEDIUM: Phosphate buffer VOLUME OF MEDIUM: 50 ml PROCESS: Complete homogenization PREVIOUS OR CURRENT METHOD: shift blender, mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked very well and desired results were achieved.

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Intestinal Samples (Swine)

PRODUCT APPLICATION REPORT

MATERIAL: Intestinal samples (Swine) VOLUME OF MATERIAL: 2mm pieces MEDIUM: VOLUME OF MEDIUM: PROCESS: Lysed PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: DOI: To obtain representative measurements from the 2-cm jejunal samples, three cross-sections of approximately 2 mm were pooled and lysed using PRO 200 Post-Mounted Laboratory http://dx.doi.org/10.1371/journal.pone.0079343

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