Thursday, January 28, 2016

#whatimhomogenizing - turfgrass

PRO Scientific high shear homogenizers are effective at breaking down tough agricultural material like turfgrass, be it for turfgrass research or biofuel research.  

 
Depending upon the sample size, we can pinpoint the exact ideal motor unit and probe, but most applications can be handled with a PRO250 homogenizer with either a 20mm or 30mm generator probe.


#whatimhomogenizing


Homogenizing is often a first step in research so we see all types of samples!
What are you homogenizing today?
Share with us what your lab is homogenizing and make sure to tag it
 #whatimhomogenizing

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Monday, January 25, 2016

Homogenizing frozen & cold samples

On this cold morning, did you know PRO Homogenizer's can homogenize frozen and cold samples?
 
Our homogenizers LOVE the cold!


Unlike sonication and bead-beating homogenization techniques, our mechanical, rotor stator generators probes can quickly and effectively process your cold or frozen sample in mere seconds without producing heat.  

Friday, January 22, 2016

1/23/16 - PRO Scientific Homogenizers peer reviewed news

We are so proud to be a part of our customer's valuable research. 
We know how hard you work on your research and publications.  





Our customers have been really busy!
Here is where PRO Homogenizers are being referenced this week, take a look...

Brain tissue - Rat
· Tissue samples were briefly homogenized with a PRO250 homogenizer in a lysis buffer cocktail containing protease and phosphatase inhibitors
· Samples were then sonicated three times for 5s each using a ultrasonicator
· This research would have benefited from using a DPS-20 which first mechanically homogenizes the sample and then processes the same sample ultrasonically
·                       PRO250 Homogenizer
·                       DPS-20 Two-Step Homogenizing System


Liver - Rat
· Total RNA extraction
· Each liver sample we homogenized in physiological saline using a PRO200 homogenizer
·               Bio-Gen PRO200 homogenizer



Cornea - Mice 
· Corneas were homogenized in an ice bath using a tissue homogenizer
·        PRO Scientific Homogenizer





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Wednesday, January 20, 2016

Tissue Homogenizer Systems


PRO offers a variety of tissue homogenizers for rapid, effective homogenization of human, animal and plant tissue samples.  

PRO Scientific Tissue Homogenizer range includes:
Hand-Held Homogenizers
Homogenizer Package Kits
Digital High-Speed Homogenizers
Programmable Homogenizing Systems
Rapid Tissue Homogenizing System
Two-Step Automated Mechanical & Ultrasonic Tissue Homogenizer Processors
See how easily and effectively PRO's Multi-Prep system homogenizes multiple tissue samples at once and in only seconds!
Learn more about tissue homogenizers
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Multi-Prep Homogenizer System 
 

Rapid Multi-Sample Tissue Homogenization System

FEATURES
  • Homogenize up to 6 samples at a time
  • Samples homogenized in seconds
  • Store up to 10 different programs
  • Avoid cross contamination
  • Learn More

Tuesday, January 19, 2016

Homogenizing from A - Z Post #2

Homogenizing A - Z 


Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters C & D here: 

Cat Fish
PRODUCT APPLICATION REPORT
MATERIAL:Cat Fish
VOLUME OF MATERIAL:Not reported
MEDIUM:None used
VOLUME OF MEDIUM:None used
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO250 Homogenizer with stand or PR0300 D Homogenizer with 473 ml gladd chamber assembly & 2" diameter blade 
APPLICATION AND RESULTS:


Successfully homogenized the Catfish.
However, some skin did get wrapped around the blade.Equipment supplied was demo equipment and the
blades may not have been as sharp as a production blade. This could have contributed to the skin wrapping around the blade.
Recommend using a sharpened blade. The entire length of the blade should be sharpened, instead of the standard tips of the blade.

Cell membrane
PRODUCT APPLICATION REPORT
MATERIAL:Cell membrane
VOLUME OF MATERIAL:50 ml
MEDIUM:PBS type
VOLUME OF MEDIUM:50 ml
PROCESS:Cell membrane preparation
PREVIOUS OR CURRENT METHOD:Large homogenizers
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Quicker and easier than other homogenizers along with achieving same results.


Cells
PRODUCT APPLICATION REPORT
MATERIAL:Cells
VOLUME OF MATERIAL:.5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM:5 ml
PROCESS:Break open cell membranes
PREVIOUS OR CURRENT METHOD:Freezing and thawing
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Completely homogenized and broken down in
fifteen seconds.


Chicken cartilage
PRODUCT APPLICATION REPORT
MATERIAL:Chicken cartilage
VOLUME OF MATERIAL:Not reported
MEDIUM:Sucrose
VOLUME OF MEDIUM:1/2 ml
PROCESS:Homogenization without destroying the sub-cellular
components
PREVIOUS OR CURRENT METHOD:Dounce homogenizer
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Ran well and kept sub-cellular components


Chicken gizzards
PRODUCT APPLICATION REPORT
MATERIAL:Chicken gizzards
VOLUME OF MATERIAL:50g
MEDIUM:Acetone
VOLUME OF MEDIUM:200 ml
PROCESS:Homogenization for chemical analysis
PREVIOUS OR CURRENT METHOD:Polytron, Tissuemizer
EQUIPMENT USED:PRO250 Homogenizer w/stand or PRO300 Homogenizer with 20mm Generator or Blade PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS:Blade system did not completely
homogenize the sample, but results were adequate enough. However,
the 20mm generator completely homogenized the sample in two
minutes after pre-chopping the gizzard into 1 cm pieces.
RECOMMENDATIONS: Cutting large chunks of tissue into pieces
will allow a greater number of applications with the
generators.


Chicken tissue
PRODUCT APPLICATION REPORT
MATERIAL:Chicken tissue
VOLUME OF MATERIAL:30 mg of tissue
MEDIUM:RLT buffer
VOLUME OF MEDIUM:600 ul
PROCESS:30s homogenization twice
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer with MULTI-GEN 7 generator
APPLICATION AND RESULTS:Successfully homogenized for RNA processing
Link DOI:http://dx.doi.org/10.1016/j.cimid.2012.02.004


Chlorophilia
PRODUCT APPLICATION REPORT
MATERIAL:Chlorophilia
VOLUME OF MATERIAL:5-10 ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Water quality testing
PREVIOUS OR CURRENT METHOD:Ultrasonic
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Worked in homogenizing the sample


Citrus bark
PRODUCT APPLICATION REPORT
MATERIAL:Citrus bark
VOLUME OF MATERIAL:5 ml
MEDIUM:Phosphate buffer
VOLUME OF MEDIUM:Not reported
PROCESS:Extraction
PREVIOUS OR CURRENT METHOD:Liquid nitrogen and break up with hammer
EQUIPMENT USED:PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS:Able to achieve desired results with both
generator sizes.


Clumped white cell agluts
PRODUCT APPLICATION REPORT
MATERIAL:Clumped white cell agluts
VOLUME OF MATERIAL:Not reported
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Steinbach homogenizer
EQUIPMENT USED:PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS:Worked well with both
generator sizes.


Corpus luteum
PRODUCT APPLICATION REPORT
MATERIAL:Corpus luteum
VOLUME OF MATERIAL:Not reported
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization for RNA extraction and RT
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:Bio-Gen PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:
The CLs were homogenized with TRIzol Reagent
http://dx/doi.org/10.1530/REP-13-0398


Cotton roots
PRODUCT APPLICATION REPORT
MATERIAL:Cotton roots
VOLUME OF MATERIAL:5-6 ml
MEDIUM:SPW
VOLUME OF MEDIUM:5-6 ml
PROCESS:Reduce in size and release hormones
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Worked well with tender roots, but not as
effective with hardened stems. For best results use a tube a
little larger in diameter than the generator


Cranberries
PRODUCT APPLICATION REPORT
MATERIAL:Cranberries
VOLUME OF MATERIAL:30 ml
MEDIUM:Water
VOLUME OF MEDIUM:20 ml
PROCESS:Homogenization of berries in robot assembly line
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO250 Homogenizer with stand or PR0300 D Homogenizer with gblade and chamber assembly
APPLICATION AND RESULTS:Worked well


Cultured bone marrow macrophagecells
PRODUCT APPLICATION REPORT
MATERIAL:Cultured bone marrow macrophage cells
VOLUME OF MATERIAL:Not reported
MEDIUM:Phosphate buffered saline
VOLUME OF MEDIUM:2.25 ml
PROCESS:Membrane suspension from whole cell suspension
PREVIOUS OR CURRENT METHOD:Ultrasound, barrel and pestle
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:With old system, only able to achieve 400
rpms of binding above the blank value of 100. With the PRO200 Homogenizer
system, achieved 4000 rpms within seconds. However, results were
too good for the experiment that was being conducted.


Drosophilia aelanogaster
PRODUCT APPLICATION REPORT
MATERIAL:Drosophilia melanogaster
VOLUME OF MATERIAL:3-10 fruit flies
MEDIUM:Aqueous buffer
VOLUME OF MEDIUM:100-300 ml
PROCESS:Complete homogenization and release of DNA for
polymerize chain-reaction
PREVIOUS OR CURRENT METHOD:Grinding with glass rod or
applicator sticks
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:








Worked well in grinding the fruit flies
and preservation of DNA, however, scientists were concerned over
the mixing of DNA molecules from different strains as material
stuck to the generators during processing.
RECOMMENDATIONS: Generators can be completely immersed and
sterilized between applications with minimal disassembly.



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Thursday, January 14, 2016

#whatimhomogenizing - Pine Needles

#whatimhomogenizing


Pine Needles

Pine Needles easily homogenized with the PRO250 Homogenizer




Homogenizing is often a first step in research so we see all types of samples!
What are you homogenizing today?
Share with us what your lab is homogenizing and make sure to tag it
 #whatimhomogenizing


Request a Quote