Homogenizing from A - Z Post #1

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters A & B here: 

Acetate PRODUCT APPLICATION REPORT MATERIAL: Acetate fiber VOLUME OF MATERIAL: 1 g MEDIUM: Methanol and Freon solvent VOLUME OF MEDIUM: 100 ml PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Oester blender EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with safety sealed generators APPLICATION AND RESULTS: Obtained needed effect in 10 seconds, 1/6 the time of the blender. Aribidopsis seedlings

PRODUCT APPLICATION REPORT

MATERIAL: 5 ml VOLUME OF MATERIAL: Aribidopsis seedlings MEDIUM: Extraction buffer VOLUME OF MEDIUM: 150 ml PROCESS: RNA extraction PREVIOUS OR CURRENT METHOD: Brinkmannn polytron EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Excellent homogenization of entire seedling after grinding with mortar and pestle. Got large yields from the PRO200 Homogenizer  and acceptable RNA samples.

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Aspirin tablets (20 tablets)

PRODUCT APPLICATION REPORT

MATERIAL: Aspirin tablets (20 tablets) VOLUME OF MATERIAL: 325mg per tablet MEDIUM: None VOLUME OF MEDIUM: None PROCESS: Homogenize to a fine powder PREVIOUS OR CURRENT METHOD: Unknown EQUIPMENT USED: cra: Test 1 & 2: 30mm generator (02-30150) Test 3 & 4: 20mm generator (02-20150) APPLICATION AND RESULTS: Various tests were run as follows: Test 1: 10,000 rpm - 1 minute moving up, down, and around in a glass jar. Test 2: 10,000 rpm - 45 seconds moving up, down, and around in a 150m1 glass beaker. Test 3: 30,000 rpm - 1 minute moving up, down, and around in a 150ml glass beaker. Test 4: 30,000 rpm - 35 seconds moving up and down in a 50ml conical tube. RECOMMENDATIONS: Test 1: fine powder with no lumps. Test 2: fine powder with no lumps. Test 3: a powder with some lumps. Test 4: very fine powder with no lumps. In all four tests, the aspirin was reduced to a powder.

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Bacteria

PRODUCT APPLICATION REPORT

MATERIAL: Bacteria VOLUME OF MATERIAL: 500ml MEDIUM: Culture VOLUME OF MEDIUM: Not reported PROCESS: Complete dispersion with low foaming PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron EQUIPMENT USED: PRO200 Homogenizer with 20mm PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: Worked perfectly with minimal foaming.

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Bacterial phase-filaments

PRODUCT APPLICATION REPORT

MATERIAL: Bacterial phage-filaments VOLUME OF MATERIAL: .015-.02 ml MEDIUM: Aqueous buffer VOLUME OF MEDIUM: 1-1.5 ml PROCESS: disruption of the filament PREVIOUS OR CURRENT METHOD: Mechanical Shear and Hand-press EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Achieved desired results in 25-30 sec.

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Biological tissue

PRODUCT APPLICATION REPORT

MATERIAL: Biological tissue VOLUME OF MATERIAL: 5ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Break down tissue for analysis and testing PREVIOUS OR CURRENT METHOD: Hand mixer EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Achieved desired results

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Bone and joint tissue

PRODUCT APPLICATION REPORT

MATERIAL: Bone and joint tissue VOLUME OF MATERIAL: Micro volumes MEDIUM: Tween-80 in TSB VOLUME OF MEDIUM: Micro volumes PROCESS: Bacteria in the bone and joint tissue were isolated PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: Achieved desired results for overnight culture

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Bovine brain

PRODUCT APPLICATION REPORT

MATERIAL: Bovine brain VOLUME OF MATERIAL: 50 g (10mm gen.) and 150 g (37mm gen.) MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron PT10 / 35 EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with 10 & 30mm generators APPLICATION AND RESULTS: Successfully homogenized the Bovine brain with both volumes and generators.

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Bovine neutrophil pellets

PRODUCT APPLICATION REPORT

MATERIAL: Bovine neutrophil pellets VOLUME OF MATERIAL: Not reported MEDIUM: TRIzol VOLUME OF MEDIUM: 1 ml PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: DOI: Achieved desired homogenization results, stored at -80°C for RNA extraction Application report

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Bovine thyroid tissue

PRODUCT APPLICATION REPORT

MATERIAL: Bovine thyroid tissue VOLUME OF MATERIAL: 50ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked better than expected.

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Bovine tongue-epithelial tissue

PRODUCT APPLICATION REPORT

MATERIAL: Bovine tongue-epithelial tumor tissue VOLUME OF MATERIAL: cut into small pieces MEDIUM: Not reported VOLUME OF MEDIUM: PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: DOI: Achieved desired homogenization results http://dx.doi.org/10.3892/mmr.2014.2887

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