Tuesday, February 21, 2017

Does it homogenize tissue?

A customer asked today...
Does it really homogenize tissue?

Absolutely!  
In tubes as small .5ml microtubes, PRO Scientific homogenizers are ideal tissue homogenizers!


Monday, February 20, 2017

Find yourself asking? What homogenizer do I need?

Find yourself asking?
What homogenizer do I need?

We at PRO Scientific know you have you a lot of choices to make in your lab, so we want to help make choosing a lab homogenizer as simple as possible.

Visit our website to review homogenizing options and let us help you find the best homoenizer for your lab's application!
https://proscientific.com/homogenizer


Tuesday, June 7, 2016

Choosing a laboratory rotor stator homogenizer

Need a homogenizer?


We make choosing a homogenizer easy! 
Just follow our helpful chart to find the package to suit your lab's needs!  All packages include everything you need to begin homogenizing. 

Tuesday, May 24, 2016

Homogenizing from A - Z Post #5

Homogenizing A - Z 


Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters K & L here: 


Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL:Keratinocytes 
VOLUME OF MATERIAL:Not reported
MEDIUM:
VOLUME OF MEDIUM:Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO200 Homogenizer
APPLICATION AND RESULTS:

DOI:
Successful homogenization
http://dx.doi.org/10.1038/ncomms6230

Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL:Psoriatic keratinocytes 
VOLUME OF MATERIAL:Not reported
MEDIUM:TRIzol reagent
VOLUME OF MEDIUM:Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO Homogenizer
APPLICATION AND RESULTS:

DOI:
Successful homogenization
http://dx.doi.org/10.1046/j.1523-1747.2002.19509.x


Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL:Dog keratinocytes 
VOLUME OF MATERIAL:Not reported
MEDIUM:RNALater
VOLUME OF MEDIUM:Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:None
EQUIPMENT USED:PRO200 Homogenizer w/Multi-Gen 7
APPLICATION AND RESULTS:

DOI:
Successful homogenization
http://dx.doi.org/10.1111/vde.12034



Kidneys
PRODUCT APPLICATION REPORT


MATERIAL:Kidneys
VOLUME OF MATERIAL:
MEDIUM:PBS
VOLUME OF MEDIUM:5 ml
PROCESS:Complete homogenization
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:PRO Homogenizer
APPLICATION AND RESULTS:


DOI:
Kidneys excised for fungal burden assessments 
were weighed, placed in 5 ml PBS, and homogenized
for 30 s with a tissue homogenizer.

http://dx.doi.org/10.1128/mBio.00723-13


Kidneys
PRODUCT APPLICATION REPORT


MATERIAL:Kidneys
VOLUME OF MATERIAL:
MEDIUM:
VOLUME OF MEDIUM:
PROCESS:For Western blotting, grinded, quantified and then homogenized. 
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:PRO Homogenizer
APPLICATION AND RESULTS:


DOI:
To investigate HSP expression, some portion of the isolated right and left kidneys were cut into
pieces and stored at −72°C. For Western blotting, the stored kidney pieces were grinded, quantified, and then homogenized. 



Larval samples
PRODUCT APPLICATION REPORT
MATERIAL:Larval samples
VOLUME OF MATERIAL:
MEDIUM:cold Tri- HCl buffer
VOLUME OF MEDIUM:4:1 v/w 
PROCESS:Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:PRO200 Homogenizer   
APPLICATION AND RESULTS:

DOI:
Larval samples were homogenized using a tissue homogenizer.
http://dx/doi.org/10.1007/s10695-013-9884-5

Latex particles
PRODUCT APPLICATION REPORT

MATERIAL:Latex particles
VOLUME OF MATERIAL:Not reported
MEDIUM:Dispersed and continuois phase 
VOLUME OF MEDIUM:Ratio of 1:3
PROCESS:Successive homogenization at 14,500 rpm for
40 seconds and 21,000 rpm for 20 seconds
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer  
APPLICATION AND RESULTS:


DOI:

Successful homogenization

http://dx.doi.org/10.1080/01932691.2014.966310


Leaves
PRODUCT APPLICATION REPORT
MATERIAL:Leaves
VOLUME OF MATERIAL:25 ml
MEDIUM:Organic buffer
VOLUME OF MEDIUM:75 ml
PROCESS:Grinding of leaves for protein analysis
PREVIOUS OR CURRENT METHOD:Brinkmannn Polytron
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Good results.


Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL:Lipid and aqueous phases
VOLUME OF MATERIAL:Not reported
MEDIUM:Lipid and aqueous phases
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO250 Homogenizer
APPLICATION AND RESULTS:Blended by a high-shear homogenizer


Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL:Lipid and aqueous phases
VOLUME OF MATERIAL:Not reported
MEDIUM:Lipid and aqueous phases
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO250 Homogenizer
APPLICATION AND RESULTS:

DOI:
Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250.

http://dx.doi.org/10.1208/s12248-013-9550-y



Liver cells from rat
PRODUCT APPLICATION REPORT
MATERIAL:Liver cells from rat
VOLUME OF MATERIAL:10 ml
MEDIUM:Krebs-Ringer buffer
VOLUME OF MEDIUM:20 ml
PROCESS:Rupturing of hepatocytes and leaving of sub-cellular
components intact
PREVIOUS OR CURRENT METHOD:Dounce homogenizer
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:The PRO200 Homogenizer  ruptured the cells in a very
short time (2 minutes) leaving the sub-cellular components
intact. There was minimal foaming and the compactness and noise
level of the instrument were added benefits

Liver tissue (mouse)
PRODUCT APPLICATION REPORT
MATERIAL:Liver tissue (mouse)
VOLUME OF MATERIAL:.5-5 ml
MEDIUM:PBS
VOLUME OF MEDIUM:Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED:PRO Homogenizer
APPLICATION AND RESULTS:

DOI:
Liver tissue was homogenized on ice for 30 s.

http://dx/doi.org/10.1021/jf403173k


Lymphocyte brain and thymus
PRODUCT APPLICATION REPORT
MATERIAL:Lymphocyte, brain, and thymus
VOLUME OF MATERIAL:.5-5 ml
MEDIUM:GITC buffer
VOLUME OF MEDIUM:Not reported
PROCESS:Isolation of RNA and DNA
PREVIOUS OR CURRENT METHOD:Mortar and pestle, blender
EQUIPMENT USED:PRO200 Homogenizer with 5mm or 7mm generator
APPLICATION AND RESULTS:Application successful
with either generator between the volumes of
material.





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Thursday, May 12, 2016

What's the key to great homogenizing?






When it comes to homogenizing, the key to homogenizing is the generator probe as this is the component that actually comes into contact with your sample.


Whether your are equipping your PRO Homogenizer with one of our PRO Quick Connect Generator Probes or our Multi-Gen 7XL Multi-Sample Generator Probes to homogenize your tissue samples, you will get consistent results each and every time.  

All PRO Generator Probes are precision crafted to ensure efficient and effective homogenization and are manufactured out of 316SS to be chemically compatible with your sample and reagents.