Homogenizing from A - Z Post #2

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters C & D here: 

Cat Fish

PRODUCT APPLICATION REPORT

MATERIAL: Cat Fish VOLUME OF MATERIAL: Not reported MEDIUM: None used VOLUME OF MEDIUM: None used PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with 473 ml gladd chamber assembly & 2" diameter blade  APPLICATION AND RESULTS: Successfully homogenized the Catfish. However, some skin did get wrapped around the blade.Equipment supplied was demo equipment and the blades may not have been as sharp as a production blade. This could have contributed to the skin wrapping around the blade. Recommend using a sharpened blade. The entire length of the blade should be sharpened, instead of the standard tips of the blade.

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Cell membrane

PRODUCT APPLICATION REPORT

MATERIAL: Cell membrane VOLUME OF MATERIAL: 50 ml MEDIUM: PBS type VOLUME OF MEDIUM: 50 ml PROCESS: Cell membrane preparation PREVIOUS OR CURRENT METHOD: Large homogenizers EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Quicker and easier than other homogenizers along with achieving same results.

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Cells

PRODUCT APPLICATION REPORT

MATERIAL: Cells VOLUME OF MATERIAL: .5 ml MEDIUM:  Not reported VOLUME OF MEDIUM: 5 ml PROCESS: Break open cell membranes PREVIOUS OR CURRENT METHOD: Freezing and thawing EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Completely homogenized and broken down in fifteen seconds.

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Chicken cartilage

PRODUCT APPLICATION REPORT

MATERIAL: Chicken cartilage VOLUME OF MATERIAL: Not reported MEDIUM: Sucrose VOLUME OF MEDIUM: 1/2 ml PROCESS: Homogenization without destroying the sub-cellular components PREVIOUS OR CURRENT METHOD: Dounce homogenizer EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Ran well and kept sub-cellular components

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Chicken gizzards

PRODUCT APPLICATION REPORT

MATERIAL: Chicken gizzards VOLUME OF MATERIAL: 50g MEDIUM: Acetone VOLUME OF MEDIUM: 200 ml PROCESS: Homogenization for chemical analysis PREVIOUS OR CURRENT METHOD: Polytron, Tissuemizer EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with 20mm Generator or Blade PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: Blade system did not completely homogenize the sample, but results were adequate enough. However, the 20mm generator completely homogenized the sample in two minutes after pre-chopping the gizzard into 1 cm pieces. RECOMMENDATIONS: Cutting large chunks of tissue into pieces will allow a greater number of applications with the generators.

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Chicken tissue

PRODUCT APPLICATION REPORT

MATERIAL: Chicken tissue VOLUME OF MATERIAL: 30 mg of tissue MEDIUM: RLT buffer VOLUME OF MEDIUM: 600 ul PROCESS: 30s homogenization twice PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with MULTI-GEN 7 generator APPLICATION AND RESULTS: Successfully homogenized for RNA processing Link DOI: http://dx.doi.org/10.1016/j.cimid.2012.02.004

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Chlorophilia

PRODUCT APPLICATION REPORT

MATERIAL: Chlorophilia VOLUME OF MATERIAL: 5-10 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Water quality testing PREVIOUS OR CURRENT METHOD: Ultrasonic EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked in homogenizing the sample

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Citrus bark

PRODUCT APPLICATION REPORT

MATERIAL: Citrus bark VOLUME OF MATERIAL: 5 ml MEDIUM: Phosphate buffer VOLUME OF MEDIUM: Not reported PROCESS: Extraction PREVIOUS OR CURRENT METHOD: Liquid nitrogen and break up with hammer EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Able to achieve desired results with both generator sizes.

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Clumped white cell agluts

PRODUCT APPLICATION REPORT

MATERIAL: Clumped white cell agluts VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Steinbach homogenizer EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Worked well with both generator sizes.

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Corpus luteum

PRODUCT APPLICATION REPORT

MATERIAL: Corpus luteum VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization for RNA extraction and RT PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: Bio-Gen PRO200 Homogenizer APPLICATION AND RESULTS: DOI: The CLs were homogenized with TRIzol Reagent http://dx/doi.org/10.1530/REP-13-0398

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Cotton roots

PRODUCT APPLICATION REPORT

MATERIAL: Cotton roots VOLUME OF MATERIAL: 5-6 ml MEDIUM: SPW VOLUME OF MEDIUM: 5-6 ml PROCESS: Reduce in size and release hormones PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked well with tender roots, but not as effective with hardened stems. For best results use a tube a little larger in diameter than the generator

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Cranberries

PRODUCT APPLICATION REPORT

MATERIAL: Cranberries VOLUME OF MATERIAL: 30 ml MEDIUM: Water VOLUME OF MEDIUM: 20 ml PROCESS: Homogenization of berries in robot assembly line PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with gblade and chamber assembly APPLICATION AND RESULTS: Worked well

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Cultured bone marrow macrophagecells

PRODUCT APPLICATION REPORT

MATERIAL: Cultured bone marrow macrophage cells VOLUME OF MATERIAL: Not reported MEDIUM: Phosphate buffered saline VOLUME OF MEDIUM: 2.25 ml PROCESS: Membrane suspension from whole cell suspension PREVIOUS OR CURRENT METHOD: Ultrasound, barrel and pestle EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: With old system, only able to achieve 400 rpms of binding above the blank value of 100. With the PRO200 Homogenizer system, achieved 4000 rpms within seconds. However, results were too good for the experiment that was being conducted.

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Drosophilia aelanogaster

PRODUCT APPLICATION REPORT

MATERIAL: Drosophilia melanogaster VOLUME OF MATERIAL: 3-10 fruit flies MEDIUM: Aqueous buffer VOLUME OF MEDIUM: 100-300 ml PROCESS: Complete homogenization and release of DNA for polymerize chain-reaction PREVIOUS OR CURRENT METHOD: Grinding with glass rod or applicator sticks EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Worked well in grinding the fruit flies and preservation of DNA, however, scientists were concerned over the mixing of DNA molecules from different strains as material stuck to the generators during processing. RECOMMENDATIONS: Generators can be completely immersed and sterilized between applications with minimal disassembly. Learn more about our homogenizers