Homogenizing from A - Z Post #5

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters K & L here: 

Keratinocytes  PRODUCT APPLICATION REPORT MATERIAL: Keratinocytes  VOLUME OF MATERIAL: Not reported MEDIUM: VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: DOI: Successful homogenization http://dx.doi.org/10.1038/ncomms6230 Keratinocytes  PRODUCT APPLICATION REPORT MATERIAL: Psoriatic keratinocytes  VOLUME OF MATERIAL: Not reported MEDIUM: TRIzol reagent VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO Homogenizer APPLICATION AND RESULTS: DOI: Successful homogenization http://dx.doi.org/10.1046/j.1523-1747.2002.19509.x Keratinocytes  PRODUCT APPLICATION REPORT MATERIAL: Dog keratinocytes  VOLUME OF MATERIAL: Not reported MEDIUM: RNALater VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer w/Multi-Gen 7 APPLICATION AND RESULTS: DOI: Successful homogenization http://dx.doi.org/10.1111/vde.12034 Kidneys PRODUCT APPLICATION REPORT MATERIAL: Kidneys VOLUME OF MATERIAL: MEDIUM: PBS VOLUME OF MEDIUM: 5 ml PROCESS: Complete homogenization PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO Homogenizer APPLICATION AND RESULTS: DOI: Kidneys excised for fungal burden assessments  were weighed, placed in 5 ml PBS, and homogenized for 30 s with a tissue homogenizer. http://dx.doi.org/10.1128/mBio.00723-13 Kidneys PRODUCT APPLICATION REPORT MATERIAL: Kidneys VOLUME OF MATERIAL: MEDIUM: VOLUME OF MEDIUM: PROCESS: For Western blotting, grinded, quantified and then homogenized.  PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO Homogenizer APPLICATION AND RESULTS: DOI: To investigate HSP expression, some portion of the isolated right and left kidneys were cut into pieces and stored at −72°C. For Western blotting, the stored kidney pieces were grinded, quantified, and then homogenized.  http://dx/doi.org/10.1016/j.transproceed.2013.08.028 Larval samples PRODUCT APPLICATION REPORT MATERIAL: Larval samples VOLUME OF MATERIAL: MEDIUM: cold Tri- HCl buffer VOLUME OF MEDIUM: 4:1 v/w  PROCESS: Homogenized PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO200 Homogenizer    APPLICATION AND RESULTS: DOI: Larval samples were homogenized using a tissue homogenizer. http://dx/doi.org/10.1007/s10695-013-9884-5 Latex particles PRODUCT APPLICATION REPORT MATERIAL: Latex particles VOLUME OF MATERIAL: Not reported MEDIUM: Dispersed and continuois phase  VOLUME OF MEDIUM: Ratio of 1:3 PROCESS: Successive homogenization at 14,500 rpm for 40 seconds and 21,000 rpm for 20 seconds PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer   APPLICATION AND RESULTS: DOI: Successful homogenization http://dx.doi.org/10.1080/01932691.2014.966310 Leaves PRODUCT APPLICATION REPORT MATERIAL: Leaves VOLUME OF MATERIAL: 25 ml MEDIUM: Organic buffer VOLUME OF MEDIUM: 75 ml PROCESS: Grinding of leaves for protein analysis PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Good results. Lipid and aqueous phases PRODUCT APPLICATION REPORT MATERIAL: Lipid and aqueous phases VOLUME OF MATERIAL: Not reported MEDIUM: Lipid and aqueous phases VOLUME OF MEDIUM: Not reported PROCESS: Homogenized for 5 minutes PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO250 Homogenizer APPLICATION AND RESULTS: Blended by a high-shear homogenizer Lipid and aqueous phases PRODUCT APPLICATION REPORT MATERIAL: Lipid and aqueous phases VOLUME OF MATERIAL: Not reported MEDIUM: Lipid and aqueous phases VOLUME OF MEDIUM: Not reported PROCESS: Homogenized for 5 minutes PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO250 Homogenizer APPLICATION AND RESULTS: DOI: Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250. http://dx.doi.org/10.1208/s12248-013-9550-y Liver cells from rat PRODUCT APPLICATION REPORT MATERIAL: Liver cells from rat VOLUME OF MATERIAL: 10 ml MEDIUM: Krebs-Ringer buffer VOLUME OF MEDIUM: 20 ml PROCESS: Rupturing of hepatocytes and leaving of sub-cellular components intact PREVIOUS OR CURRENT METHOD: Dounce homogenizer EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: The PRO200 Homogenizer  ruptured the cells in a very short time (2 minutes) leaving the sub-cellular components intact. There was minimal foaming and the compactness and noise level of the instrument were added benefits Liver tissue (mouse) PRODUCT APPLICATION REPORT MATERIAL: Liver tissue (mouse) VOLUME OF MATERIAL: .5-5 ml MEDIUM: PBS VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO Homogenizer APPLICATION AND RESULTS: DOI: Liver tissue was homogenized on ice for 30 s. http://dx/doi.org/10.1021/jf403173k Lymphocyte brain and thymus PRODUCT APPLICATION REPORT MATERIAL: Lymphocyte, brain, and thymus VOLUME OF MATERIAL: .5-5 ml MEDIUM: GITC buffer VOLUME OF MEDIUM: Not reported PROCESS: Isolation of RNA and DNA PREVIOUS OR CURRENT METHOD: Mortar and pestle, blender EQUIPMENT USED: PRO200 Homogenizer with 5mm or 7mm generator APPLICATION AND RESULTS: Application successful with either generator between the volumes of material.

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