Homogenizing from A - Z Post #3

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters E, F & G here: 

Ecoli cells

PRODUCT APPLICATION REPORT

MATERIAL: E. Coli cells VOLUME OF MATERIAL: 25 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Breakage of cell (100%) PREVIOUS OR CURRENT METHOD: Ultrasonic homogenizer EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Did not get 100% breakage in 15 seconds. Sometimes longer running times are needed to obtain complete homogenization. Fifteen seconds is not quite long enough for the type of disruption needed for this application.

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Escherichia coil

PRODUCT APPLICATION REPORT

MATERIAL: Escherichia coli VOLUME OF MATERIAL: Not reported MEDIUM: Chemical solution VOLUME OF MEDIUM: Not reported PROCESS: Isolation of fimbriae PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 20mm generator APPLICATION AND RESULTS: The 20mm generator produced the required homogenization result.

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Fall Armyworm

PRODUCT APPLICATION REPORT

MATERIAL: Fall Armyworm VOLUME OF MATERIAL: MEDIUM: 4 ml of phosphate buffered saline VOLUME OF MEDIUM: 15 ml tube PROCESS: Homogenized PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO Homogenizer  APPLICATION AND RESULTS: DOI: Individual speciments were homogenized in 4 ml of phosphate buffered saline in a 15ml test tube using a tissue homogenizer. http://dx.doi.org/10.1653/024.096.0305

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Fig Newton cookies

PRODUCT APPLICATION REPORT

MATERIAL: Fig Newton cookies VOLUME OF MATERIAL: 20 grams MEDIUM: None VOLUME OF MEDIUM: Not reported PROCESS: Grinding to measure moisture PREVIOUS OR CURRENT METHOD: Waring blender EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: Able to grind the Fig Newton, but lack of medium caused the paste within the cookie to become heated and reduces the moisture content, which had comparable results to the blender. A medium is recommended.

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Fish oil

PRODUCT APPLICATION REPORT

MATERIAL: Fish oil VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenize for future application PREVIOUS OR CURRENT METHOD: Mechanical and ultrasonic homogenizer EQUIPMENT USED: PRO250 Homogenizer with speed control box APPLICATION AND RESULTS: DOI: The hot emulsion resulted by mixing the lipid and aqueous phases at 85°C was exposed to an external mechanical energy by high shear homogenization. http://www.scientificbulletin.upb.ro/rev_docs_arhiva/rezc5e_246325.pdf

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Fish tissue

PRODUCT APPLICATION REPORT

MATERIAL: Fish tissue VOLUME OF MATERIAL: 10-400 ml MEDIUM: Water VOLUME OF MEDIUM: Not reported PROCESS: Homogenize for future application PREVIOUS OR CURRENT METHOD: Hand and ultrasonic homogenizer EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: Worked well and sealed system proved to be essential success of application.

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Food additives

PRODUCT APPLICATION REPORT

MATERIAL: Food additives VOLUME OF MATERIAL: various MEDIUM: : 1) Mayonnaise 2) Salad dressing 1 gallon (PRO200 Homogenizer  Process) VOLUME OF MEDIUM: 1 gallon PROCESS: Mix food product while adding various additives in liquid and powder form to obtain vertical flow. PREVIOUS OR CURRENT METHOD: Waring blender EQUIPMENT USED: PRO250 Homogenizer w/stand & 20mm probe or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: The PRO200 Homogenizer  and the 20mm generator were unable to mix the mayonnaise and the secret additive, however it worked superbly on the salad dressing. The PRO300 D Homogenizer and the blade system were used on the mayonnaise and began to mix but there was no vertical flow of material. RECOMMENDATIONS: Recommend that a larger diameter generator and PRO250 Homogenizer or PRO300 D Homogenizer be used to cause vertical flow of the material.

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Food staples

PRODUCT APPLICATION REPORT

MATERIAL: Food samples VOLUME OF MATERIAL: 1 ml MEDIUM: Organic aqueous VOLUME OF MEDIUM: 5 ml PROCESS: Complete dispersion PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Good homogenization results

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Freshwater snail

PRODUCT APPLICATION REPORT

MATERIAL: Freshwater snail VOLUME OF MATERIAL: Not reported MEDIUM: distilled water VOLUME OF MEDIUM: 0.5 ml PROCESS: Homogenized PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO200 Homogenizer   APPLICATION AND RESULTS: DOI: Each body was weighed, transferred to a flat bottom tube and homogenized for 15 s. http://dx/doi.org/10.1080/00288330.2013.846921

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Frog embryos

PRODUCT APPLICATION REPORT

MATERIAL: Frog embryos VOLUME OF MATERIAL: Not reported MEDIUM: varied VOLUME OF MEDIUM: 1.5 ml PROCESS: Cell disruption PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Worked well.

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Frozen rat heart

PRODUCT APPLICATION REPORT

MATERIAL: Frozen rat heart VOLUME OF MATERIAL: 130-150 mg MEDIUM: HEPES, EDTA, DTT, MgCl2 VOLUME OF MEDIUM: 25-30mM PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: Achieved desired results Link DOI: http://dx.doi.org/10.1002/jcp.24079

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Fungi

PRODUCT APPLICATION REPORT

MATERIAL: 50 ml VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: 50 ml PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked well.

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Gypsy Moth eggs

PRODUCT APPLICATION REPORT

MATERIAL: Gypsy Moth eggs VOLUME OF MATERIAL: 05 ml MEDIUM: Aqueous VOLUME OF MEDIUM: 1 ml PROCESS: Rupture of eggs PREVIOUS OR CURRENT METHOD: Manual EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Successfully ruptured eggs for further analysis.

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