#whatimhomogenizing - turfgrass

PRO Scientific high shear homogenizers are effective at breaking down tough agricultural material like turfgrass, be it for turfgrass research or biofuel research.  

 

Depending upon the sample size, we can pinpoint the exact ideal motor unit and probe, but most applications can be handled with a PRO250 homogenizer with either a 20mm or 30mm generator probe.

#whatimhomogenizing

Homogenizing is often a first step in research so we see all types of samples! What are you homogenizing today?

Share with us what your lab is homogenizing and make sure to tag it  #whatimhomogenizing

Visit our choosing a lab homogenizer page. View all of our PRO Lab Homogenizer Units »

Homogenizing frozen & cold samples

On this cold morning, did you know PRO Homogenizer's can homogenize frozen and cold samples?

 

Our homogenizers LOVE the cold!

Unlike sonication and bead-beating homogenization techniques, our mechanical, rotor stator generators probes can quickly and effectively process your cold or frozen sample in mere seconds without producing heat.  

Visit our choosing a lab homogenizer page. View all of our PRO Lab Homogenizer Units »

1/23/16 - PRO Scientific Homogenizers peer reviewed news

We are so proud to be a part of our customer's valuable research. 

We know how hard you work on your research and publications.  

Our customers have been really busy!

Here is where PRO Homogenizers are being referenced this week, take a look...

Brain tissue - Rat

· Tissue samples were briefly homogenized with a PRO250 homogenizer in a lysis buffer cocktail containing protease and phosphatase inhibitors

· Samples were then sonicated three times for 5s each using a ultrasonicator

· This research would have benefited from using a DPS-20 which first mechanically homogenizes the sample and then processes the same sample ultrasonically

·                       PRO250 Homogenizer

·                       DPS-20 Two-Step Homogenizing System

 Molecular and Cellular Neuroscience Bulletin

Liver - Rat

· Total RNA extraction

· Each liver sample we homogenized in physiological saline using a PRO200 homogenizer

·               Bio-Gen PRO200 homogenizer

Food and Function

Cornea - Mice 

· Corneas were homogenized in an ice bath using a tissue homogenizer

·        PRO Scientific Homogenizer

 The American Journal of Pathology

Visit our choosing a lab homogenizer page. View all of our PRO Lab Homogenizer Units »

Tissue Homogenizer Systems

PRO offers a variety of tissue homogenizers for rapid, effective homogenization of human, animal and plant tissue samples.

PRO Scientific Tissue Homogenizer range includes: Hand-Held Homogenizers Homogenizer Package Kits Digital High-Speed Homogenizers Programmable Homogenizing Systems Rapid Tissue Homogenizing System Two-Step Automated Mechanical & Ultrasonic Tissue Homogenizer Processors

See how easily and effectively PRO's Multi-Prep system homogenizes multiple tissue samples at once and in only seconds! Learn more about tissue homogenizers Request a quote Multi-Prep Homogenizer System    Rapid Multi-Sample Tissue Homogenization System FEATURES Homogenize up to 6 samples at a time Samples homogenized in seconds Store up to 10 different programs Avoid cross contamination Learn More

Homogenizing from A - Z Post #2

Homogenizing A - Z 

Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters C & D here: 

Cat Fish

PRODUCT APPLICATION REPORT

MATERIAL: Cat Fish VOLUME OF MATERIAL: Not reported MEDIUM: None used VOLUME OF MEDIUM: None used PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with 473 ml gladd chamber assembly & 2" diameter blade  APPLICATION AND RESULTS: Successfully homogenized the Catfish. However, some skin did get wrapped around the blade.Equipment supplied was demo equipment and the blades may not have been as sharp as a production blade. This could have contributed to the skin wrapping around the blade. Recommend using a sharpened blade. The entire length of the blade should be sharpened, instead of the standard tips of the blade.

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Cell membrane

PRODUCT APPLICATION REPORT

MATERIAL: Cell membrane VOLUME OF MATERIAL: 50 ml MEDIUM: PBS type VOLUME OF MEDIUM: 50 ml PROCESS: Cell membrane preparation PREVIOUS OR CURRENT METHOD: Large homogenizers EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Quicker and easier than other homogenizers along with achieving same results.

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Cells

PRODUCT APPLICATION REPORT

MATERIAL: Cells VOLUME OF MATERIAL: .5 ml MEDIUM:  Not reported VOLUME OF MEDIUM: 5 ml PROCESS: Break open cell membranes PREVIOUS OR CURRENT METHOD: Freezing and thawing EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Completely homogenized and broken down in fifteen seconds.

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Chicken cartilage

PRODUCT APPLICATION REPORT

MATERIAL: Chicken cartilage VOLUME OF MATERIAL: Not reported MEDIUM: Sucrose VOLUME OF MEDIUM: 1/2 ml PROCESS: Homogenization without destroying the sub-cellular components PREVIOUS OR CURRENT METHOD: Dounce homogenizer EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Ran well and kept sub-cellular components

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Chicken gizzards

PRODUCT APPLICATION REPORT

MATERIAL: Chicken gizzards VOLUME OF MATERIAL: 50g MEDIUM: Acetone VOLUME OF MEDIUM: 200 ml PROCESS: Homogenization for chemical analysis PREVIOUS OR CURRENT METHOD: Polytron, Tissuemizer EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with 20mm Generator or Blade PRO Safety Sealed Chamber Assembly APPLICATION AND RESULTS: Blade system did not completely homogenize the sample, but results were adequate enough. However, the 20mm generator completely homogenized the sample in two minutes after pre-chopping the gizzard into 1 cm pieces. RECOMMENDATIONS: Cutting large chunks of tissue into pieces will allow a greater number of applications with the generators.

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Chicken tissue

PRODUCT APPLICATION REPORT

MATERIAL: Chicken tissue VOLUME OF MATERIAL: 30 mg of tissue MEDIUM: RLT buffer VOLUME OF MEDIUM: 600 ul PROCESS: 30s homogenization twice PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with MULTI-GEN 7 generator APPLICATION AND RESULTS: Successfully homogenized for RNA processing Link DOI: http://dx.doi.org/10.1016/j.cimid.2012.02.004

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Chlorophilia

PRODUCT APPLICATION REPORT

MATERIAL: Chlorophilia VOLUME OF MATERIAL: 5-10 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Water quality testing PREVIOUS OR CURRENT METHOD: Ultrasonic EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked in homogenizing the sample

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Citrus bark

PRODUCT APPLICATION REPORT

MATERIAL: Citrus bark VOLUME OF MATERIAL: 5 ml MEDIUM: Phosphate buffer VOLUME OF MEDIUM: Not reported PROCESS: Extraction PREVIOUS OR CURRENT METHOD: Liquid nitrogen and break up with hammer EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Able to achieve desired results with both generator sizes.

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Clumped white cell agluts

PRODUCT APPLICATION REPORT

MATERIAL: Clumped white cell agluts VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Steinbach homogenizer EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator APPLICATION AND RESULTS: Worked well with both generator sizes.

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Corpus luteum

PRODUCT APPLICATION REPORT

MATERIAL: Corpus luteum VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization for RNA extraction and RT PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: Bio-Gen PRO200 Homogenizer APPLICATION AND RESULTS: DOI: The CLs were homogenized with TRIzol Reagent http://dx/doi.org/10.1530/REP-13-0398

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Cotton roots

PRODUCT APPLICATION REPORT

MATERIAL: Cotton roots VOLUME OF MATERIAL: 5-6 ml MEDIUM: SPW VOLUME OF MEDIUM: 5-6 ml PROCESS: Reduce in size and release hormones PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked well with tender roots, but not as effective with hardened stems. For best results use a tube a little larger in diameter than the generator

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Cranberries

PRODUCT APPLICATION REPORT

MATERIAL: Cranberries VOLUME OF MATERIAL: 30 ml MEDIUM: Water VOLUME OF MEDIUM: 20 ml PROCESS: Homogenization of berries in robot assembly line PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with gblade and chamber assembly APPLICATION AND RESULTS: Worked well

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Cultured bone marrow macrophagecells

PRODUCT APPLICATION REPORT

MATERIAL: Cultured bone marrow macrophage cells VOLUME OF MATERIAL: Not reported MEDIUM: Phosphate buffered saline VOLUME OF MEDIUM: 2.25 ml PROCESS: Membrane suspension from whole cell suspension PREVIOUS OR CURRENT METHOD: Ultrasound, barrel and pestle EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: With old system, only able to achieve 400 rpms of binding above the blank value of 100. With the PRO200 Homogenizer system, achieved 4000 rpms within seconds. However, results were too good for the experiment that was being conducted.

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Drosophilia aelanogaster

PRODUCT APPLICATION REPORT

MATERIAL: Drosophilia melanogaster VOLUME OF MATERIAL: 3-10 fruit flies MEDIUM: Aqueous buffer VOLUME OF MEDIUM: 100-300 ml PROCESS: Complete homogenization and release of DNA for polymerize chain-reaction PREVIOUS OR CURRENT METHOD: Grinding with glass rod or applicator sticks EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Worked well in grinding the fruit flies and preservation of DNA, however, scientists were concerned over the mixing of DNA molecules from different strains as material stuck to the generators during processing. RECOMMENDATIONS: Generators can be completely immersed and sterilized between applications with minimal disassembly. Learn more about our homogenizers

#whatimhomogenizing - Pine Needles

#whatimhomogenizing

Pine Needles

Pine Needles easily homogenized with the PRO250 Homogenizer

Homogenizing is often a first step in research so we see all types of samples! What are you homogenizing today? Share with us what your lab is homogenizing and make sure to tag it  #whatimhomogenizing Visit our choosing a lab homogenizer page. View all of our PRO Lab Homogenizer Units »