|
| MATERIAL: | Keratinocytes |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: |
|
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: |
|
| PREVIOUS OR CURRENT METHOD: | None |
| EQUIPMENT USED: | PRO200 Homogenizer |
APPLICATION AND RESULTS:
DOI: | Successful homogenization
http://dx.doi.org/10.1038/ncomms6230
|
|
|
| MATERIAL: | Psoriatic keratinocytes |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: | TRIzol reagent |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: |
|
| PREVIOUS OR CURRENT METHOD: | None |
| EQUIPMENT USED: | PRO Homogenizer |
APPLICATION AND RESULTS:
DOI: | Successful homogenization
http://dx.doi.org/10.1046/j.1523-1747.2002.19509.x
|
|
|
| MATERIAL: | Dog keratinocytes |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: | RNALater |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: |
|
| PREVIOUS OR CURRENT METHOD: | None |
| EQUIPMENT USED: | PRO200 Homogenizer w/Multi-Gen 7 |
APPLICATION AND RESULTS:
DOI: | Successful homogenization
http://dx.doi.org/10.1111/vde.12034
|
|
Kidneys |
|
| MATERIAL: | Kidneys |
| VOLUME OF MATERIAL: |
|
| MEDIUM: | PBS |
| VOLUME OF MEDIUM: | 5 ml |
| PROCESS: | Complete homogenization |
| PREVIOUS OR CURRENT METHOD: |
|
| EQUIPMENT USED: | PRO Homogenizer |
APPLICATION AND RESULTS:
DOI: | Kidneys excised for fungal burden assessments
were weighed, placed in 5 ml PBS, and homogenized
for 30 s with a tissue homogenizer.
http://dx.doi.org/10.1128/mBio.00723-13 |
|
|
| MATERIAL: | Kidneys |
| VOLUME OF MATERIAL: |
|
| MEDIUM: |
|
| VOLUME OF MEDIUM: |
|
| PROCESS: | For Western blotting, grinded, quantified and then homogenized. |
| PREVIOUS OR CURRENT METHOD: |
|
| EQUIPMENT USED: | PRO Homogenizer |
APPLICATION AND RESULTS:
DOI: | To investigate HSP expression, some portion of the isolated right and left kidneys were cut into
pieces and stored at −72°C. For Western blotting, the stored kidney pieces were grinded, quantified, and then homogenized.
|
|
|
| MATERIAL: | Larval samples |
| VOLUME OF MATERIAL: |
|
| MEDIUM: | cold Tri- HCl buffer |
| VOLUME OF MEDIUM: | 4:1 v/w |
| PROCESS: | Homogenized |
| PREVIOUS OR CURRENT METHOD: |
|
| EQUIPMENT USED: | PRO200 Homogenizer |
APPLICATION AND RESULTS:
DOI: | Larval samples were homogenized using a tissue homogenizer.
http://dx/doi.org/10.1007/s10695-013-9884-5
|
|
|
| MATERIAL: | Latex particles |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: | Dispersed and continuois phase |
| VOLUME OF MEDIUM: | Ratio of 1:3 |
| PROCESS: | Successive homogenization at 14,500 rpm for
40 seconds and 21,000 rpm for 20 seconds |
| PREVIOUS OR CURRENT METHOD: | Not reported |
| EQUIPMENT USED: | PRO200 Homogenizer |
APPLICATION AND RESULTS:
DOI:
| Successful homogenization
http://dx.doi.org/10.1080/01932691.2014.966310 |
|
Leaves |
|
| MATERIAL: | Leaves |
| VOLUME OF MATERIAL: | 25 ml |
| MEDIUM: | Organic buffer |
| VOLUME OF MEDIUM: | 75 ml |
| PROCESS: | Grinding of leaves for protein analysis |
| PREVIOUS OR CURRENT METHOD: | Brinkmannn Polytron |
| EQUIPMENT USED: | PRO200 Homogenizer with 10mm generator |
| APPLICATION AND RESULTS: | Good results. |
|
|
| MATERIAL: | Lipid and aqueous phases |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: | Lipid and aqueous phases |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: | Homogenized for 5 minutes |
| PREVIOUS OR CURRENT METHOD: | Not reported |
| EQUIPMENT USED: | PRO250 Homogenizer |
| APPLICATION AND RESULTS: | Blended by a high-shear homogenizer |
|
|
| MATERIAL: | Lipid and aqueous phases |
| VOLUME OF MATERIAL: | Not reported |
| MEDIUM: | Lipid and aqueous phases |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: | Homogenized for 5 minutes |
| PREVIOUS OR CURRENT METHOD: | Not reported |
| EQUIPMENT USED: | PRO250 Homogenizer |
APPLICATION AND RESULTS:
DOI: | Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250.
http://dx.doi.org/10.1208/s12248-013-9550-y
|
|
|
| MATERIAL: | Liver cells from rat |
| VOLUME OF MATERIAL: | 10 ml |
| MEDIUM: | Krebs-Ringer buffer |
| VOLUME OF MEDIUM: | 20 ml |
| PROCESS: | Rupturing of hepatocytes and leaving of sub-cellular
components intact |
| PREVIOUS OR CURRENT METHOD: | Dounce homogenizer |
| EQUIPMENT USED: | PRO200 Homogenizer with 10mm generator |
| APPLICATION AND RESULTS: | The PRO200 Homogenizer ruptured the cells in a very
short time (2 minutes) leaving the sub-cellular components
intact. There was minimal foaming and the compactness and noise
level of the instrument were added benefits
|
|
Liver tissue (mouse) |
|
| MATERIAL: | Liver tissue (mouse) |
| VOLUME OF MATERIAL: | .5-5 ml |
| MEDIUM: | PBS |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: |
|
| PREVIOUS OR CURRENT METHOD: |
|
| EQUIPMENT USED: | PRO Homogenizer |
APPLICATION AND RESULTS:
DOI: | Liver tissue was homogenized on ice for 30 s.
http://dx/doi.org/10.1021/jf403173k
|
|
| Lymphocyte brain and thymus |
|
| MATERIAL: | Lymphocyte, brain, and thymus |
| VOLUME OF MATERIAL: | .5-5 ml |
| MEDIUM: | GITC buffer |
| VOLUME OF MEDIUM: | Not reported |
| PROCESS: | Isolation of RNA and DNA |
| PREVIOUS OR CURRENT METHOD: | Mortar and pestle, blender |
| EQUIPMENT USED: | PRO200 Homogenizer with 5mm or 7mm generator |
| APPLICATION AND RESULTS: | Application successful
with either generator between the volumes of
material. |
|
|
