PRO Homogenizers are ideal for RNA / DNA isolation

RNA DNA Homogenizer

PRO Homogenizers are ideal for RNA / DNA isolation

Sample disruption is a necessary early step in the isolation of RNA, DNA, and proteins.  PRO Scientific rotor stator homogenizers are an ideal physical method used to disrupt the sample since thorough homogenization of cells or tissues is an essential step in RNA isolation that prevents both RNA loss and RNA degradation.  The method of homogenization should always be tailored to the cell or tissue type, whereas most cultured cells can be homogenized by simply vortexing in a cell lysis solution, animal tissues, plant tissues, yeast, and bacteria often require more rigorous methods of disruption that a PRO homogenzier can provide. 

PRO rotor-stator homogenizer generator probes are precision crafted with a very narrow clearance between the inner rotating shaft and outer tube/chamber.  This allows for quick, effective and repetitive processing, meaning you will get the results you want each and every time. 

The variable speed of our homogenizers allows for the utmost control of your homogenization to ensure a high yield and fragment size. 

Our multi-sample homogenizing systems such as the Multi-Gen 7XL, DPS-20 and Multi-Prep ensure sample purity by avoiding cross contamination between samples.

Regardless of which PRO homogenizer setup is selected, most homogenization can be completed in a matter of seconds which eliminates heat production and ensures preservation of the sample.

Here are just some of the many application references of RNA / DNA isolation and extraction with a PRO Scientific Homogenizer:

RNA Isolation and Nuclease Protection, total RNA was isolated from whole organs – PRO200 Homogenizer

RNA isolation, two groups of four hippocampi – PRO200 Homogenizer

RNA isolation of aorta – PRO200 Homogenizer

RNA isolation frozen tissue – PRO200 Homogenizer

RNA isolation aorta (walls eptum and right ventricle) – PRO200 Homogenizer

RNA isolation of human spermatozoal – PRO200 Homogenizer with Multi-Gen 7

RNA extraction from chicken melanoma tissue – Multi-Gen 7

DNA isolation, entire muscles of mice and rats and monkeys – PRO200 Homogenizer

DNA isolation of liver – PRO200 Homogenizer

DNA isolation organs – PRO250 Homogenizer

DNA isolation mycobacterium tuberculosis – Multi-Gen 7

DNA isolation rat uterus – Multi-Gen 7

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Homogenizer Selection Guide

Factors to think about when selecting a homogenizer

Wet or Dry Sample

Wet Samples - with some sort of liquid - best in generator system.

·        Liquid allows for more cavitational breakdown.

·        PTFE bearings in generators work best with some sort of liquid in sample.

Dry Samples - when no liquids are present - best with blade system.

(Or custom bearings for non-liquid samples may be substituted)

Sample Size

Generators are capable of homogenizing a sample size from 0.03ml to 20L.

(Larger non-viscous samples possible with deflector head attachment)

·        5mm or 7mm generators can fit into 1.5ml - 2ml eppendorf tubes.

o   With a 7mm generator, the generator probe won’t touch the bottom of conical microtubes, but substance may get pulled up into generator.  The generator will touch the bottom of a flat bottom microtube.

 

The sample, if solid, should be no longer than half the diameter of the generator.

Generator Width

Generally you want to homogenize and use a generator as wide as the tube you are using.

·        This will allow for faster and better homogenizing because no material will "escape" from the generator.

·        Keep in mind that there is some displacement of material once the generator enters the tube or container.

We have pre-selected homogenizer packages available that are ideal for processing in specific tube size.

Micro Homogenizer Packages

Micro Sample Homogenizing - Ideal for .5-2ml tubes

Standard

Deluxe

Premium

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Multi-Gen 7XL Homogenizing

Multi-Sample Homogenizing - Ideal for 1.5-50ml tubes

Premium

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MaX-Homogenizer Packages

Tube to Beaker Homogenizing - Ideal for 15-50ml tubes & small-medium beakers

Standard

Deluxe

Premium

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Universal Homogenizer Packages

Full Tube Range Homogenizing Ideal for 1.5-50ml tubes

Standard

Deluxe

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Multi-Gen 7 Homogenizer Package

Multi-Sample Homogenizing - Ideal for 1.5-5ml tubes

Standard

Deluxe

Premium

Larger tubes and vessels can also be accommodated with PRO Quick Connect Generators from 20mm to 59mm in diameter.

  

Location of Generator Within the Sample

Place the generator off-center in the tube or container for the best results.

• ·        This will prevent material from getting "stuck" on the walls of the tube and not being homogenized.
  ·        Place the generator 1/3 of the sample height off of the bottom of the tube or container.
  
  
  
  
  Configuration of Container & Generator Length
  When using a generator 120mm or greater in length, it is important to remember that these generators have a mid-bearing. This “bearing” helps prevent bending or movement of the rotor within the tube.

This mid-bearing still needs to be kept moist during homogenizing and therefore should be kept covered by the substance being homogenized, not doing this will burn out the bearing, produce excessive amounts of heat and damage your generator. This will also cause the sample to become dingy and blackened.

If using too long of a generator for your sample size, you may start to pump air because the generator cannot recover fast enough.

Use as short a generator you can for your container/tube.

Review our Homogenizer Generator Probe Maintenance page for additional homogenizing tips and tricks.

Speed and Particle Size Reduction

Always begin homogenizing at a low speed, then gradually increase speed. This allows you to remain in control of the whole process.

Peripheral speed (tip speed of the generator) which is affected by the size of the container and the size of the generator is more important then

RPM. The peripheral speed is directly proportional to the amount of cavitational breakdown. Cavitational breakdown allows for smaller particle

reduction. PRO homogenizers are capable of reducing particles to 0.01 microns when cavitational breakdown is present.

Most homogenizing can be done in 15 - 30 seconds if not you may be:

1. ·        Using the wrong size generator
2. ·        Not locating the generator properly in the container
3. ·        Too viscous of a material

PRO Scientific offers a variety of economical homogenizer packages which have been preselected to better assist you in choosing a homogenizer setup.

Click here to select a homogenizer package that best suit your needs.

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Evolving Products in the Homogenizing Field

As technology moves forward in laboratory research and develop­ment, new products are emerg­ing within the field of sample prepara­tion. One of the continuously evolving product lines is homogenizing equipment. As technology advances, the industry will rely on novel developments within this field. Some of these developments, offered by PRO Scientific (Oxford, CT), are described below.

Dual processing system

The DPS-20 dual processing system combines mechanical and ultrasonic homogenizing methods in a closed com­pact and timesaving unit with full automa­tion. The system permits the use of each homogenizing method, dependently or independently of each other. It has the memory capacity to store up to nine pro­grams at one time; alternatively, the sys­tem can be used in manual mode for indi­vidual sample processing. Users are given maximum control to best support their own processing needs.

 Generator probes

Viewed as the better alternative to plastic generator probes, which are often seen as disposable, the Multi-Gen 7 is a 7-mm-diam generator probe designed to meet the ever-increasing demand to homogenize numerous samples without the risk of cross-contamination, and it eliminates the need to spend valuable time cleaning the generator between samples. The probes are designed to quickly lock into any of the manufacturer’s homogenizer units through the Multi-Gen Adapter. The adapter permits hands-free ejection of the used generator probe, which minimizes the chance of accidental contamination when attaching the next generator probe.

Mechanical benchtop homogenizers

Responding to the needs for mechanical homogenizers that can accurately and repet­itively perform batch processing, two lines of high-quality benchtop homogenizers, the D-Series and PC-Series, were developed. The slim profile of both homogenizer lines provides a more modern visual appeal, while their technological abilities are equally impressive. Each line consists of four units under the model type PRO300, PRO350, PRO400, and PRO400EL (with a conve­nient extra-long stand). Both series include the PRO TRAC motorized stand, which enables effortless, accurate, and smooth motor positioning. With sample volume range capability from 0.03 mL to 30 L, the homogenizers are well suited for homog­enizing, emulsifying, blending, and/or mix­ing organic and inorganic materials.

Homogenization of biohazardous materials

There is currently an increasing demand to safely homogenize biohazardous materi­als. Safety Seal tubes and chamber assem­blies were designed to provide a two-way barrier to keep both the end user and the sample safe. The assemblies protect end users from hazardous aerosols that are generated during processing. Delicate or precious samples are also carefully iso­lated from external contaminants. The assemblies prevent the user from oper­ating the blade system while the blade is exposed, thus providing an additional level of safety; the tube or chamber must be assembled correctly for the generator or blade to run. The assemblies are avail­able in tube, glass, or stainless steel, rang­ing from volumes of 1.5 mL to 1 L. All the assemblies work with the manufacturer’s homogenizers, from handheld to benchtop models. Safety Seal tubes and chamber assemblies are an extremely safe method of homogenizing, with either a blade for large, solid material sample processing or a rotor stator generator probe for smaller solid material or liquid processing.

View all of our PRO Homogenizer Units.

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Bead Mill Homogenizer vs. PRO Homogenizer

Bead Mill Homogenizer vs. PRO Homogenizer

True or False?

“Bead mills provide better cell lysis compared to rotor stator homogenizers.”

False:

Mechanical homogenizers are more than capable of providing the same level of cell lysis in a fraction of the time it takes a bead mill. 

Most mechanical homogenizing is done in a matter of seconds with ZERO heat produced and you can continue on with your same tubes you are processing in, no need to buy special tubes and beads! 

The same piece of PRO homogenizing equipment can homogenize both soft and hard tissue samples.

 

Bead mill homogenizers were developed for samples too tough to be homogenized in a common lab vortex mixer. 

However, they are not the answer for most mechanical homogenizing needs that require anything stronger than a lab blender results.

PRO Scientific Homogenizers can successfully process a WIDE RANGE of samples including but not limited to:

Muscle tissue

Cardiac tissue

Bone sample

Food samples including pork and tough materials

Plant material

 

Bead mill homogenizing is self-contained in a custom bead mill tube…however this mean you are either restricted with the type of tubes you can continue working in your sample preparation or you have to transfer your sample.

PRO Scientific Homogenizers allow you to economically homogenize in your current tube or containers.
We have generator probes to accommodate tubes from .5ml up to 50ml and every size in between!

 

With a bead mill there are a variety of beads are available dependent upon your sample type…however this creates a consumable commodity and your lab must be constantly stocked with a variety of costly beads.

Homogenizing should not be a consumable product.
Your lab budget should be saved for other necessary pieces of equipment and supplied.
With our PRO Homogenizers, you buy what you need once and that is it!
Our PRO Homogenizer Packages are on sale and have been pre-selected to best suit your particular application needs.

 

 

PRO Generators are precision crafted with a very narrow clearance between the inner rotating shaft and outer tube/chamber. This allows for quick, effective and repetitive processing, meaning you will get the results you want each and every time!

Our PRO generator probes can allow you to homogenize down to a submicron level if needed.

PRO Rotor Stator Homogenizers provide better results compared to Bead Mill Homogenizers

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