Wednesday, January 13, 2016

#whatimhomogenizing

What are you homogenizing? 


Homogenizing is often a first step in research so we see all types of samples!
What are you homogenizing today?
Share with us what your lab is homogenizing and make sure to tag it
 #whatimhomogenizing

Here's what we've been homogenizing lately:
Pine Needles #whatimhomogenizing








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Homogenizing from A - Z Post #1



Homogenizing A - Z 


Homogenizing is one of the first steps in research and PRO homogenizers have been used to process so many different type of samples from A (acetate) to Z (zebrasfish).

What can we help you homogenize today?
Take a look at our extensive homogenizing application database

Or review letters A & B here: 


Acetate
PRODUCT APPLICATION REPORT
MATERIAL:Acetate fiber
VOLUME OF MATERIAL:1 g
MEDIUM:Methanol and Freon solvent
VOLUME OF MEDIUM:100 ml
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Oester blender
EQUIPMENT USED:PRO250 Homogenizer with stand or PR0300 D Homogenizer with safety sealed generators
APPLICATION AND RESULTS:Obtained needed effect in 10 seconds, 1/6 the time of the blender.


Aribidopsis seedlings
PRODUCT APPLICATION REPORT
MATERIAL:5 ml
VOLUME OF MATERIAL:Aribidopsis seedlings
MEDIUM:Extraction buffer
VOLUME OF MEDIUM:150 ml
PROCESS:RNA extraction
PREVIOUS OR CURRENT METHOD:Brinkmannn polytron
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Excellent homogenization of entire seedling after grinding with mortar and pestle. Got large yields from the PRO200 Homogenizer  and acceptable RNA samples.



Aspirin tablets (20 tablets)
PRODUCT APPLICATION REPORT
MATERIAL:Aspirin tablets (20 tablets)
VOLUME OF MATERIAL:325mg per tablet
MEDIUM:None
VOLUME OF MEDIUM:None
PROCESS:Homogenize to a fine powder
PREVIOUS OR CURRENT METHOD:Unknown
EQUIPMENT USED:cra:
Test 1 & 2: 30mm generator (02-30150)
Test 3 & 4: 20mm generator (02-20150)
APPLICATION AND RESULTS:Various tests were run as follows:

Test 1: 10,000 rpm - 1 minute moving up, down, and around in a glass jar.

Test 2: 10,000 rpm - 45 seconds moving up, down, and around in a 150m1 glass beaker.

Test 3: 30,000 rpm - 1 minute moving up, down, and around in a 150ml glass beaker.
Test 4: 30,000 rpm - 35 seconds moving up and down in a 50ml conical tube.

RECOMMENDATIONS:
Test 1: fine powder with no lumps.
Test 2: fine powder with no lumps.
Test 3: a powder with some lumps.
Test 4: very fine powder with no lumps. In all four tests, the aspirin was reduced to a powder.


Bacteria
PRODUCT APPLICATION REPORT
MATERIAL:Bacteria
VOLUME OF MATERIAL:500ml
MEDIUM:Culture
VOLUME OF MEDIUM:Not reported
PROCESS:Complete dispersion with low foaming
PREVIOUS OR CURRENT METHOD:Brinkmannn Polytron
EQUIPMENT USED:PRO200 Homogenizer with 20mm PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS:Worked perfectly with minimal foaming.


Bacterial phase-filaments
PRODUCT APPLICATION REPORT
MATERIAL:Bacterial phage-filaments
VOLUME OF MATERIAL:.015-.02 ml
MEDIUM:Aqueous buffer
VOLUME OF MEDIUM:1-1.5 ml
PROCESS:disruption of the filament
PREVIOUS OR CURRENT METHOD:Mechanical Shear and Hand-press
EQUIPMENT USED:PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS:Achieved desired results in 25-30 sec.


Biological tissue
PRODUCT APPLICATION REPORT
MATERIAL:Biological tissue
VOLUME OF MATERIAL:5ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Break down tissue for analysis and testing
PREVIOUS OR CURRENT METHOD:Hand mixer
EQUIPMENT USED:PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS:Achieved desired results


Bone and joint tissue
PRODUCT APPLICATION REPORT
MATERIAL:Bone and joint tissue
VOLUME OF MATERIAL:Micro volumes
MEDIUM:Tween-80 in TSB
VOLUME OF MEDIUM:Micro volumes
PROCESS:Bacteria in the bone and joint tissue were isolated
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer
APPLICATION AND RESULTS:Achieved desired results for overnight culture


Bovine brain
PRODUCT APPLICATION REPORT
MATERIAL:Bovine brain
VOLUME OF MATERIAL:50 g (10mm gen.) and 150 g (37mm gen.)
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Brinkmannn Polytron PT10 / 35
EQUIPMENT USED:PRO250 Homogenizer with stand or PR0300 D Homogenizer with 10 & 30mm generators
APPLICATION AND RESULTS:Successfully homogenized the Bovine brain
with both volumes and generators.


Bovine neutrophil pellets
PRODUCT APPLICATION REPORT
MATERIAL:Bovine neutrophil pellets
VOLUME OF MATERIAL:Not reported
MEDIUM:TRIzol
VOLUME OF MEDIUM:1 ml
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:
Achieved desired homogenization results,
stored at -80°C for RNA extraction
Application report


Bovine thyroid tissue
PRODUCT APPLICATION REPORT
MATERIAL:Bovine thyroid tissue
VOLUME OF MATERIAL:50ml
MEDIUM:Not reported
VOLUME OF MEDIUM:Not reported
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS:Worked better than expected.


Bovine tongue-epithelial tissue

PRODUCT APPLICATION REPORT
MATERIAL:Bovine tongue-epithelial tumor tissue
VOLUME OF MATERIAL:cut into small pieces
MEDIUM:Not reported
VOLUME OF MEDIUM:
PROCESS:Homogenization
PREVIOUS OR CURRENT METHOD:Not reported
EQUIPMENT USED:PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:
Achieved desired homogenization results
http://dx.doi.org/10.3892/mmr.2014.2887

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Thursday, December 10, 2015

Homogenization of creams, lotions, vegetable glycerin or some other viscous material


PRO Scientific deflector heads are specifically designed to enhance the homogenization of larger volume and higher viscosity materials such as creams, lotions, oils, gels and much more.  They are used regularly by cosmetic, nutrition, paint and oil companies.
PRO Deflector Head
The deflector head design creates vertical movement of heavy materials which would otherwise stand stagnant, and promotes increased flow on larger volume samples.
Deflector Head Sample Movement


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Friday, December 4, 2015

12/04/15 - PRO Scientific Homogenizers peer reviewed news


We are so proud to be a part of our customer's valuable research. 
We know how hard you work on your research and publications.  




Our customers have been really busy!
Here is where PRO Homogenizers are being referenced this week, take a look...

Freeze dried sprouts in acidified cold methanol
· SMCSO content was determined following a previous determined SOP  
· Two hundred grams of freeze-dried powder were extracted overnight in 30 ml acidified cold methanol
· After homogenization by a high-speed PRO Scientific Homogenizerssamples were incubated at
Liophylized sprouts
· 200 mg of the liophylized samples were extracted with 5 ml hot methanol 70 % for 5 min at
80 °C, 
· Homogenized using a high-speed PRO400 tissue homogenizer 
· Centrifuged at 5,000 rpm for 20 min using a Hettich centrifuge EBA20

    Tumor - Hepatocellular carcinoma 
    · Tissues harvested from experimental animals were homogenized with a handheld tissue homogenizer, Bio-Gen PRO200 in RIPA buffer

      Essential oils - water oil emulsions  
      ·  W/O (water/oil) emulsion was formed between organic solution with TDI oregano/sage oil and aqueous solution of 1% PVA tween 80 as a stabilizer. 
      · The emulsification was carried out at 6000 rpm for 30 min with PRO benchtop homogenizer 
      · Subsequently HDMI was added, the stirring speed was decreased to 600 rpm.